![]() Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology. Detecting Low Abundance Proteins in Western BlottingĮLISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones.Overview of Post-Translational Modification.Carbonyl-Reactive Crosslinker Chemistry.Sulfhydryl-Reactive Crosslinker Chemistry.Polyethylene Glycol (PEG) and Pegylation of Proteins.Overview of Crosslinking and Protein Modification.Metabolic Labeling and Chemoselective Ligation.Sample Preparation for Mass Spectrometry.Methods for Detecting Protein–RNA Interactions.Methods for Detecting Protein-DNA Interactions.Overview of Protein-Nucleic Acid Interactions.Label Transfer Protein Interaction Analysis.Crosslinking Protein Interaction Analysis.Overview of Protein–Protein Interaction Analysis.Immunofluorescence Method for IHC Detection.Avidin-Biotin Complex Method for IHC Detection.Immunohistochemistry vs Immunocytochemistry.Spike and Recovery and Linearity of Dilution Assessmentįactors Affecting Signal Generation in ELISA Chromogenic Western Blotting Substrates.Blocking Buffers for Western Blot and ELISA.Protein-Protein Variation of Protein Assays.Desalting and Gel Filtration Chromatography.Overview of dialysis, desalting, buffer exchange and protein concentration.Overview of the Immunoprecipitation (IP) Technique.His-tagged Proteins–Production and Purification. ![]() GST-tagged Proteins–Production and Purification.Covalent Immobilization of Affinity Ligands.Overview of Protease and Phosphatase Inhibition for Protein Preparation.Overview of Cell Fractionation and Organelle Isolation.Detergents for Cell Lysis and Protein Extraction.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |